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The dissertation in question focuses on the implementation of a simulation model developed for a project of the company Engineering Ingegneria Informatica: a vaccine supply chain. The thesis highlights how nowadays tackling complex problems has become one of the main challenges for different types of industries. Simulationbased tools are valuable solutions to deal with complex systems, especially when traditional methodologies fail to support company in their decision-making processes. By modeling and replicating real-world system characteristics and behaviors, simulation models enable what-if analysis in free-risk environments. These capabilities allow companies to anticipate potential disruptive events, identify critical problems, and make informed corrective decisions. The simulation-based tool of the case study was developed with the AnyLogic software, a leading simulation modeling software for business application, used worldwide by over 40% of Fortune 100 companies. It supports the three foundational simulation methodologies: agent-based, discrete event, and system dynamics. The developed tool offered the company the ability to simulate different supply chain configurations and scenarios. Its outputs provided a granular level of details, till to the single batch production, across a 10-year simulation horizon. This allowed decision-makers to perform in-depth analyses and effectively optimize strategic plans.